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. 2019 May 8;10:501. doi: 10.3389/fphar.2019.00501

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Copyright © 2019 Yang, Liu, Lu, Xu, Zheng, Li, Cui, Wang, Zhang, Xu, Zhou, Wang, Chen and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The P2X4R mRNA and protein levels in the hippocampus were not altered after LPS and/or Fast green FCF treatments. (A) The level of hippocampal P2X4R mRNA transcription after LPS and/or Fast green FCF treatment. GAPDH was served as internal standard. (B) The level of hippocampal P2X4R protein expression after LPS and/or Fast green FCF treatment. β-actin was served as internal standard. Fast green FCF, FGF. Data are expressed as fold change (normalized to control) and presented as means ± SE. Two–way ANOVA followed by Turkey’s post hoc test (mRNA: n = 10 mice/group; protein: n = 3 mice/group).