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. 2019 Feb 19;6(1):e000294. doi: 10.1136/lupus-2018-000294

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Serum does not cause mitochondrial dysfunction. (A) The cellular mitochondrial profile of humanumbilical vein endothelial cells in different cell culture conditions defined by the use of the inhibitors, oligomycin (oligo), FCCP and antimycin A (AntiA). (B) The bioenergetic health index of endothelial cells in buffer (2% fetal bovine serum), healthy control serum (50% v/v), or SLE serum (50% v/v), calculated based on the cellular mitochondrial profile. Analysis was conducted using a one-way analysis of variance and Fisher’s least significance difference post-test. BHI, bioenergetic health index; FCCP, trifluoromethoxy carbonylcyanide phenylhydrazone; OCR, oxygen consumption rate.