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. 2019 Feb 19;6(1):e000294. doi: 10.1136/lupus-2018-000294

Figure 6.

Figure 6

Lupus serum induces neutrophil adhesion to the endothelial cell surface. (A) Humanumbilical vein endothelial cells (HUVECs) were stimulated for 6 hours with 10% serum for 6 hours and stimulated cells were exposed to neutrophils isolated from fresh human blood as outlined in the ‘Materials and methods’ section. images are representative of 6/8 SLE sera and 4/5 control samples. Images represent calcein AM (green) stained neutrophils (first column), bright field +calcein AM stained (second column) of HUVEC cells exposed to endothelial basal medium 2 (EBM-2) (control), healthy serum, or SLE serum in the presence or absence of L-sepiapterin, 40× magnification. (B) Graph represents fluorescence intensity ±SEM. Quantification of neutrophils are reported as means±SEM n=9 SLE, five controls. *p<0.05 compared with EBM-2,++p<0.01 and+++p<0.001 compared with EBM-2 +L-sepiapterin. All data were analysed using two-way analysis of variance and Fisher’s least significant difference post-test.