Fig. 5.

Loss of GNL3L or GNL2 causes G2/M arrest with minimal DNA damage. MDA-MB-231 cells were treated with GNL3L-specific (siG3L, A–C) or GNL2-specific (siGNL2, D–F) siRNA duplexes at 100 nM for 48 hours. (A,D) Western blots of GNL3L, GNL2 and γ-H2AX. Protein loading was controlled by the amount of α-tubulin (Tub). The relative amount of the siRNA-targeted protein is indicated beneath the blots. (B,E) FACS-based quantification of the percentage of γ-H2AX⁺ cells in siRNA-treated samples. (C,F) Cell-cycle profiles and quantitative analyses (n = 4) of siRNA-treated MDA-MB-231 cells. Data represent the mean±s.e.m.