Fig. 7.

Overexpression of nucleostemin, but not of GNL3L or GNL2, protects against hydroxyurea (HU)-induced DNA damage. (A) MDA-MB-231 cells treated with siScr or siNS were tested for their clonogenic survival following treatment with HU, H₂O₂ or UV. (B) Upper panel, the percentage of γ-H2AX⁺ cells in the transfected (GFP-positive) populations of MDA-MB-231 cells transiently overexpressing GFP, nucleostemin (NS)–GFP, NSdB–GFP (a nucleoplasmic nucleostemin mutant), GNL3L–GFP or GNL2–GFP, with or without a 24-hour HU treatment. Lower panel, the percentage of γ-H2AX⁺ cells in the non-transfected (GFP-negative) populations. Differences were analyzed by repeated measures ANOVA. Data represent the mean±s.e.m.