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. Author manuscript; available in PMC: 2020 Apr 18.
Published in final edited form as: Cell. 2019 Apr 4;177(3):608–621.e12. doi: 10.1016/j.cell.2019.03.026

Figure 7. Pkd1 heterozygosity protects against CCl4 induced necrosis and fibrosis.

Figure 7.

A. Schema for Pkd1 experiments. AAV-TBG-Cre (5×1010) was injected intravenously into Pkdfl/+ mice to delete one Pkd1 allele in the liver. AAV-TBG-GFP was injected to generate control mice. Two weeks later, mice were injected with one dose of CCl4 to induce injury. In a separate experiment, 12 weeks of biweekly CCl4 was used to induce chronic damage and liver fibrosis.

B. Serum ALT and AST in Pkd1 het mice 24 hours after CCl4 injection (n = 11 and 11).

C. Necrotic cells (circled in yellow) in Pkd1 heterozygous livers 48 hours post CCl4 injection. Scale bar: 200μm.

D. Quantification of necrosis 48 hours post CCl4 injection (n = 11 and 11).

E. Sirius Red staining of liver sections after 12 weeks of biweekly CCl4 injections. Scale bar: 500pm.

F. Quantification of Sirius Red staining after chronic CCl4 injury (n = 9 and 9).

All data are presented as mean ± SEM. *, p<0.05, **, p<0.01.