TABLE 2.
Ability of various agents to alter binding of 125I-Bantag-1 to hBRS-3 receptor
hBRS-3 stably transfected Balb 3T3 cells (1 × 106 cells/ml) were incubated with 50 pM 125I-Bantag-1 either alone or with the indicated concentration of the unlabeled peptide listed. Results are expressed as the percentage of the saturable binding with no unlabeled peptide. Results are the mean ± S.E.M. from at least three experiments, and in each experiment each point was determined in duplicate.
| Agent Added | 125I-Bantag-1 Bound |
|---|---|
| % Control | |
| Peptide-1 (10 µM) | 8.50 ± 1.20 |
| Bantag-1 (0.01 nM) | 80.0 ± 4.1 |
| Bantag-1 (10 µM) | 0 |
| MK-5046 (10 µM) | 62.5 ± 3.4 |
| Bn (10 µM) | 96.0 ± 9.2 |
| CCH Amide I (10 µM) | 87.0 ± 4.3 |
| CCH Amide II (10 µM) | 99.0 ± 5.2 |
| Substance P (10 µM) | 93.0 ± 4.0 |
| CCK-8 (10 µM) | 100.0 ± 3.2 |
| Gastrin-1 (10 µM) | 94.0 ± 2.6 |
| Somatostatin-14 (10 µM) | 100.0 ± 3.3 |
| [Tyr4, D-Phe12]Bn (1 µM) | 98 ± 3.8 |
| [Leu13,Ψ(CH2NH), Leu14]Bn-(6-14) (1 µM) | 100.0 ± 4.1 |
| [D-Phe6, Leu13,Ψ(CH2NH), Cpa14]Bn-(6-14) (10 µM) | 100.0 ± 2.9 |
| [D-Phe6]Bn-(6-13)methyl ester (1 µM) | 96.3 ± 1.2 |
| [D-Phe6]Bn-(6-13)ethyl ester (1 µM) | 100.0 ± 4.2 |
| PD-1676252 (1 µM) | 99.1 ± 3.0 |
CCH, carbamylcholine; CCK, cholecystokin.