Figure 2.

2 populations of vGlut1⁺ and vGlut2⁺ DH neurons project to RSC. (a) Schematic of Cre-dependent AAV-DIO-hM4D(Gi)-mCherry infusion in DH of vGlut1-Cre and vGlut2-Cre mice (left). Labeling of vGlut1⁺ (middle) and vGlut2⁺ (right) DH neurons 6 weeks after virus expression. The mCherry labeling patterns were similar to the one of endogenous transporters (Supplementary Fig. 2). (b) Immunofluorescent staining for mCherry RSC (top) in vGlut1-Cre mice and vGlut2-Cre mice revealing terminal fields in RSC layers 1 and 3. Immunostaining in DH is shown below. (c) Retrograde labeling of DH neurons projecting to RSC using Fluoro-Gold (top and bottom left) or CTB (top and bottom right). Fluoro-Gold injected into RSC (top left) was detected predominantly in the subiculum (SUB, bottom left). Sparse signals were also found in dorsomedial CA1. Similarly, injection of CTB (blue) in RSC labels SUB neurons of vGlut1-Cre mice expressing Cre-dependent retrograde AAV-flex-dtTomato (red) (bottom left).