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Cortical interneurons are reduced in Nkx2-1-Cre; Sp9F/LacZ; Rosa-YFP conditional mutants (Sp9-CKOs). (A–E′) GFP and interneuron markers (PV, SST, CR, and NPY) double-immunostaining on P30 cortex sections. (F) Quantification showing that Sp9-CKOs had reduced GFP+, GFP+/PV+, and GFP+/SST+ cells, and had increased GFP+/NPY+ cells in the primary motor cortex. Student’s t-test, *P < 0.05; **P < 0.01, ***P < 0.001, n = 3. Scale bar: 50 μm.
