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Lhx6, Lhx8, and Shh expression were downregulated in the Sp9-CKO MGE. (A–I′) In situ RNA hybridization staining for Lhx6, Lhx8, and Shh on E12.5 3 serial coronal hemisections (rostral-most on the left) showed reduced Lhx6 expression in the MGE SVZ (arrows in B, B′), reduced Lhx8 expression in the dorsal MGE SVZ (arrows in E–F′) and reduced Shh expression in the MGE mantle zone (MZ) of Sp9-CKOs (arrows in H–H′). Cx, cortex. Scale bar: 200 μm.
