Table 2.
List of h20S proteasome subunits identified by LC separation followed by ISD. The average theoretical and experimental MW, deviation in the measured mass, sequence tags obtained, and the modifications observed for each protein are tabulated.
| UniProt ID | Subunit | Average Theoretical MW (Da) |
Average Experimental MW (Da) |
Error in mass accuracy (ppm) |
Sequence tag obtained from NSD |
Modification |
|---|---|---|---|---|---|---|
| P25786 | α1 | 29597.7 | 29597.8 | 3.38 |
249PADEPA254 260PME262 3RNQ5 |
+Nacetyl Metini |
| P25787 | α2 | 25809.4 | 25809.39 | 0.39 |
5YSFSLTT11 8SLTTFS13 |
−Metini +Nacetyl Ala1 |
| P25788 | α3 | 28424.1 | 28423.9 | 7.04 | Co-eluting protein |
−Metini +Nacetyl Ser1 +[PO3]− |
| P25789 | α4 | 29394.7 | 29395.04 | 11.57 |
33CLGI36 239HEEEEA244 |
−Metini +Nacetyl Ser1 |
| P28066 | α5 | 26453.1 | 26452.8 | 11.34 |
13NTFSPEGR20 23QVEYAIEAI31 36TAIGIQT42 39GIQT42 218ATVQP222 |
+Nacetyl Metini |
| P60900 | α6 | 27310.3 | 27310.32 | 0.73 |
2RGSSAG7 10RHITIFS16 12ITIFS16 238LVAL241 235DAH237 219VTVENP224 |
−Metini +Nacetyl Ser1 |
| O14818 | α7 | 27797.7 | 27798.1 | 14.39 | Co-eluting protein |
−Metini +Nacetyl Ser1 |
| P20618 | β1 | 23549.0 | 23548.3 | 29.73 | Co-eluting protein | No modification |
| P49721 | β2 | 22878.3 | 22878.32 | 0.87 |
2EYLIGI7 195SFPK198 |
+Nacetyl Metini |
| P49720 | β3 | 22859.8 | 22859.81 | 0.44 |
146YGMCESLWEP155 185IVH187 186VHIIEKDK193 |
−Metini +Nacetyl Ser1 |
| P28070 | β4 | 24391.8 | 24391.6 | 8.20 | 2QNPMVT7 | No modification |
| P28074 | β5 | 22458.4 | 22458.3 | 4.45 | Co-eluting protein | No modification |
| P28072 | β6 | 21903.9 | 21903.7 | 9.13 | 2TIMAVQF8 | No modification |
| Q99436 | β7 | 25295.04 | 25295.05 | 0.4 | 229QTM231 | No modification |