Fig 5. Substrate range of PruAA5_2A.

PruAA5_2A activity was measured using 300 mM substrate, except for glyceraldehyde, acetaldehyde and glycolaldehyde dimer, where the substrate concentration was 50 mM. Activity on Glyoxalic acid was measured at 15 mM since no activity was detected at 50 mM. In all cases, reactions were performed at 30°C in 20 mM MOPS (pH 7.5). n = 4; error bars indicate standard deviation.