Fig 6. Kinetic analysis of PruAA5_2A on preferred substrates.

Raffinose (■), galactose (●), glycerol (▲) and a fresh solution of glycolaldehyde dimer (▼). n = 4; error bars indicate standard deviations. The data were fitted to the Michaelis-Menten or substrate-inhibition (glycolaldehyde dimer) functions using the OriginPro analysis software (iteration algorithm: Levenberberg-Marquardt); in all cases R² values were > 0.95. For all substrates besides the glycolaldehyde dimer, saturation kinetics were not achieved below substrate solubility. Accordingly, apparent kinetic parameters are reported.