Figure 2.
Cox 2 immunohistochemical staining
Figure contains immunohistochemical staining for Cox2 showed a wide spectrum of cytoplasmic reactivity ranging from no staining (A), weak staining (B) to diffuse strong staining (C) in the lesional adenoma cells. 15-PDGH immunostain was scored as loss of staining when cytoplasmic positivity was present only in the surface epithelium (D; red arrow) with no staining in the adenoma crypt epithelium (D; blue arrows). Note the positive staining in the stromal inflammatory cells (D) that served as an internal positive control. 15-PGDH was scored as positive when there was staining observed in >1% of cells in both surface and crypt epithelium and the intensity in these cases ranged from moderate (E) to strong (F).
