Video 5. Branched microtubule networks generated with varying augmin and TPX2 concentrations.

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DOI: 10.7554/eLife.43890.016

Branched MT networks were generated in Xenopus egg extracts with 10 μM RanQ69L for four conditions: buffer addition or control depletion, addition of 260 nM TPX2, partial depletion of TPX2 (20% of control), or partial depletion of augmin (20% of control). MTs are labeled with Cy5-tubulin (red) and their plus-ends with EB1-mCherry (pseudo-colored as green). Scale bars, 10 μm. Representative movies are displayed, where time-point 0 s represents the start of the reaction.