Table 1. Summary of the evaluated conditions in the optimization in terms of number of protein identifications (PSM-, peptide- and protein level FDR 1%).

Experiment	HiRIEF pH range	Depleted plasma (mg)	# PSM	# Peptides	# Proteins
MS runtime control	NA	0.072	148304	2588	241
1	3.0–10.0	1.0	123262	15424	1626
2		0.6	118983	16372	1791
3	3.7–4.9	1.0	72287	8566	1517
4	3.7–4.05	0.2	25033	3230	1068
5		0.6	25472	3871	1394
6		1.0	39351	5421	1774
7	4.0–4.25	0.2	29224	2916	904
8		0.6	47470	4629	1534
9		1.0	64970	6012	1888
10		1.0	46230	4695	1360
11		2.0	53323	5324	1659
12		4.0	71727	6339	1812
13		6.0	70376	6149	1608
14	4.2–4.45	0.2	30067	2992	913
15		0.6	41619	4470	1422
16		1.0	63329	6042	1803
A549 cell line*	3.0–10.0	---	367570	141071	9816
A549 cell line*	3.7–4.9	---	314305	93329	9679

*Cell line data from Zhu et al. (2018).