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Conditional Tbk1-NKO mouse generation and genotyping. (A) Tbk1-NKO mice were established by crossing Tbk1fl/fl mice with Nestin-cre mice, and genotyped by PCR. (B) Western blot expression of Tbk1 in the cortex (co), cerebellum (cb), and liver (li). (C) Tbk1 immunohistochemistry in brain cortex. Bar = 20 μm.
