. 2019 Apr 21;11(8):2343–2351. doi: 10.18632/aging.101918

Table 2. Sequences of primers used for MS-qPCR for Foxp3.

Primers for detection of	Primer type	Sequence (5′ to 3′)
Methylated FOXP3-promoter	Sense	CTCTTCTCTTCCTCCGTAATATCG
Methylated FOXP3-promoter	Antisense	GTTATTGACGTTATGGCGGTC
Demethylated FOXP3-promoter	Sense	CCCTCTTCTCTTCCTCCATAATATCA
Demethylated FOXP3-promoter	Antisense	TTTTGTTATTGATGTTATGGTGGTT
Methylated FOXP3-enhancer	Sense	GTAAAGGGTAGTTGGAAGGTAAAGC
Methylated FOXP3-enhancer	Antisense	AGAGGTTTAAAAAGTGGGAGATTTC
Demethylated FOXP3-enhancer	Sense	GTACGAACCTCACACGACGA
Demethylated FOXP3-enhancer	Antisense	TAAAGGGTAGTTGGAAGGTAAAGTG
Methylated FOXP3-CpG	Sense	TACACATACAAACCTCACACAACAA
Methylated FOXP3-CpG	Antisense	ATTAACTCGCTACAACCATTATCGT
Demethylated FOXP3-CpG	Sense	AGAGGTTTAAAAAGTGGGAGATTTT
Demethylated FOXP3-CpG	Antisense	TTAACTCACTACAACCATTATCATC