Skip to main content
. Author manuscript; available in PMC: 2019 Nov 20.
Published in final edited form as: Biochemistry. 2018 Nov 5;57(46):6538–6550. doi: 10.1021/acs.biochem.8b00987

Figure 5.

Figure 5.

Nup358 competes efficiently with Rab6GTP for binding of BicD2-CTD. (A) Purified Nup358-RBD2 and BicD2-CTD were mixed in a 1:1 molar ratio and analyzed by size exclusion chromatography. An SDS-PAGE of the elution fractions is shown. Masses of molecular weight standards are shown on the left and elution volumes on the bottom. (B) The same analysis was performed for Rab6GTP and BicD2-CTD. (C) In a competition assay, Nup358-RBD2, Rab6GTP, and BicD2-CTD were mixed in a 1:1:1 molar ratio and the mixture was analyzed by size exclusion chromatography. (D−F) As controls, the individual proteins were analyzed: (D) Nup358-RBD2, (E) Rab6GTP, and (F) BicD2-CTD.