Table 1.

Table 1A Affinity (KD) measurements by ITC establish RanGTP as a regulator for the Nup358/BicD2 interaction
Cargo	BicD2	Affinity (μM)	# Sites
Nup358-RBD2	BicD2-CTD	0.5 ± 0.07	1.0
RanGTP/Nup358-RBD2	BicD2-CTD	3.8 ± 0.4	1.1
Table 1B Affinity of Rab6GTP to BicD2
Cargo	BicD2	Affinity (μM)	# Sites
Rab6GTP	BicD2-CTD	12.6 ± 1.2	1.1
Rab6GTPmin	BicD2-CTD	12 ± 1.2	1.5
Table 1C Determination of the enthalpy change ΔH and the entropy change ΔS by ITC
Cargo	BicD2	ΔH (kcal/mol)	ΔS (cal/mol/deg)
Nup358-RBD2	BicD2-CTD	−14.9	−21
RanGTP/Nup358-RBD2	BicD2-CTD	−12.8	−18
Rab6GTP	BicD2-CTD	−11.0	−13