Table 3B.

Number of BicD2 molecules recruited to cargo

Condition (Table 3A)	# BicD2 bound to Nup358	# Rab6GTP bound to BicD2
1	1000	250
2	150	250
3	0	250
4	1000	0
5	150	0

The total number of BicD2 molecules per cell was calculated to be 13000 (16nM) (Table 2).