A) Blood glucose was assessed in mice at 1200h, food was withdrawn at 0800h. B) Glucose tolerance test, 2g/kg ip was performed in overnight fasted mice at 0900h. C) Insulin tolerance test, 1.5U/kg ip was peformed in mice at 1200h, food was withdrawn at 0800h. Open circle, discontinuous line (control diet), black squares, black line (t10c12-CLA diet), grey triangles, grey line (c9t11-CLA diet). Data are represented as means +/− SEM, and analyzed by two-way ANOVA (A-C) or one-way ANOVA (D-E) followed by a Tukey posthoc analysis. Letters indicate significant differences between groups within a time point: a, control vs t10c12-CLA; b, control vs c9t11-CLA; c, t10c12-CLA vs c9t11-CLA. p<0.05. Asterisks indicate differences between control and t10c12-CLA, **, p<0.01. N= 6-12mice/group.