Figure 1. Split and pool synthesis of a DNA-encoded library.

A. Schematic depiction of a two cycle DEL using three building blocks at each position to create nine unique species. In general, the size of libraries created by split and pool chemistry will be x^y, where x = the number of unique building blocks used at that position and y = the number of cycles used to create the library. B. The general structure of an 800 million compound library containing a central triazine core. The structure of a screening hit against p38 MAP kinase (the identity of the unit shown in pink was not relevant to binding).