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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Dev Biol. 2019 Feb 5;451(1):79–85. doi: 10.1016/j.ydbio.2019.02.001

Figure 3: E2f1 is required for normal terminal cell growth and branching.

Figure 3:

Terminal cell clones were positively labeled by GFP expression (green) while tracheal tubes were visualized by autofluorescence in the UV channel (blue), and cell junctions were marked by staining against adherens junction (AJ) components (Arm, red). In control terminal cells (A-A”), a ring of AJ staining marks a single intercellular junction with a neighboring (unlabeled) autocellular stalk cell. In E2f1 mutant cells (B-B”), smaller and less branched terminal cells were connected to single stalk cells by two or more cell junctions 61% (n=28) of the time (C). Data are representative of marked homozygous mutant tracheal terminal cells examined in multiple animals from three replicate crosses to generate genetic mosaic animals. Scale bar = 10 μm.