Figure 5: The molecular identities of sprout, denuded and asthmatic.

A) Mapping and whole genome sequencing revealed a nonsense mutation within the coding sequence of InR in sprout mutant animals. The mutation is predicted to cause a truncation of the protein within the kinase domain and is therefore likely to be an amorphic allele. B) Mapping and whole genome sequencing of mutations in denuded revealed a nonsense mutation within the coding sequence of dATAD3A. The mutation is predicted to truncate the protein after 222 amino acids, completely deleting the AAA domain. C) Mutations previously assigned to the asthmatic complementation group were mapped by meiotic recombination and failed to complement mutations in zif. Sequencing of zif revealed missense mutations in the second zinc finger domain of the two of the mutations in the asthmatic complementation group – see details below. For further analysis of zif/asthmatic, we used the putative null allele, 1L15, that was previously shown to carry a non-sense mutation predicted to truncate the protein such that all zinc finger domains would be lacking.