Snord116 +/− displayed impairments in learning and memory in cued but not contextual fear conditioning. Learning and memory was evaluated by Pavlovian fear conditioning using two components, contextual cues and an auditory tone, with assessments of freezing time before and after the presentation of three tone-shock pairings. (A) Normal levels of freezing post-training indicate associations between tone and shock were made in all genotypes. In addition, both genotypes showed typical basal levels of freezing suggesting no confounds of deficits in sensory reactivity or pain threshold. (B) The 24-hr contextual component illustrated typical levels freezing, indicating no abnormal contextual learning and memory in either Snord116 + /+ or Snord116 +/− mice. (C) Cued conditioning freeze time 48-hr after initial training, before and after, the presentation of three tone-cues is illustrated. Snord116 +/− mice had lower freezing scores compared to Snord116 +/+ following the auditory reminder tone cue. *p < 0.05 versus Snord116 +/+ by student’s unpaired t-tests.