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The expression of tdTomato (A, C and D) and GFP (B) from AAV-retro vectors at the injection sites. Panel C and D showed the injection site at rostral (superior colliculus) level and caudal (inferior colliculus) level, respectively. In the vlPAG of wild type C57BL/6 mice, tdtomato expression was absent (E) while a few GFP positive cells were spotted. scale bar = 100 μm. AQ: aqueduct. mPFC: medial prefrontal cortex. vlPAG: ventrolateral periaqueductal gray.
