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. 2019 May 15;4:17. doi: 10.1038/s41541-019-0111-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Segmentation ICCS analysis of HA colocalization with Rab proteins and MHC II. a HA colocalization (number of colocalized particles per µm²—left) and colocalized fraction of HA (right) with Rab5 (top), Rab11 (middle), and Rab7 (bottom) are presented. Based on 3 or more experiments for each condition. b HA colocalization (number of colocalized particles per µm²—left) and colocalized fraction of HA (right) with MHC II are presented. Based on three experiments. Bar graphs present mean ± standard deviation (S.D.). **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA followed by Tukey’s multiple comparisons post-test)