Fig. 3.

Immunostaining and single-cell RNA-sequencing of CTCs. a With CD45 and Hoechst staining, the CD45 positive nucleated cells were identified as leukocytes. (Scale bar: 15 µm) b tSNE plot of all CTCs from patient samples processed by Hydro-Seq. (666 CTCs from 21 patient samples with each color representing individual patient sample.) c The reproducibility test of Hydro-Seq processing. For the two tubes of blood drawn from the same patient and processed on the same day, comparable CTC counts were achieved from the two experiments (Exp. 1: 13 CTCs with red color; Exp. 2: 12 CTCs with cyan color). The expression profiles of housekeeping, cell proliferation, epithelial, mesenchymal, and other genes are consistent, showing good reproducibility. Source data are provided as a Source Data file