Table 2.
Hydrodynamic parameters of PTPN3-PDZ derived from the analysis of analytical ultracentrifugation.
| PTPN3-PDZ | ||||||
|---|---|---|---|---|---|---|
| 10 μM | 25 μM | 70 μM | 70 μM + HBVc PBM | |||
| MW theoretical (kDa) | 10.5 | 10.5 | 10.5 | 12.0 | ||
| MW estimated (kDa) | 12.4 | 11.3–25.9a | nc | 13.5 | ||
| Sed coef (S0,w,20) (S) | 1.5 | 1.5–2.6a | nc | 1.6 | ||
| PTPN3-PDZ Next | ||||||
| 17.5 μM | 35 μM | 70 μM | 70 μM + HPV16E6 PBM | 70 μM + HPV18E6 PBM | 70 μM + HBVc PBM | |
| MW theoretical (kDa) | 12.7 | 12.7 | 12.7 | 14.1 | 14.1 | 14.2 |
| MW estimated (kDa) | 12.7 | 12.5 | 15 | 13.7 | 14.7 | 15.6 |
| Sed coef (S0,w,20) (S) | 1.6 | 1.7 | 1.6 | 1.6 | 1.6 | 1.6 |
aTwo peaks are present; nc: not calculated because of a continuum of species.