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. 2019 May 15;5:95. doi: 10.1038/s41420-019-0177-3

Fig. 5. The ERK1/2 pathway contributes to ROS generation in response to secretory granule permeabilization.

Fig. 5

a-f Effect of tryptase and cell signaling pathways on mefloquine-induced ROS generation in mast cells. Mast cells were preincubated ± either nafamostat mesylate (NAFA; 1 μM), NF-κB Activation Inhibitor (NF-κB inh; 0.1 μM), MK2206 (1 μM), SR11302 (10 μM), SB203580 (10 μM), or U0126 (10 μM) for 2 h, followed by incubation of cells ± mefloquine (20 μM). Cellular levels of ROS were assessed by flow cytometry after 30 min. The data shown represent means ± SEM (n = 3−4)