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. 2019 May 15;5:95. doi: 10.1038/s41420-019-0177-3

Fig. 6. Granzyme B contributes to ROS generation in response to secretory granule permeabilization.

Fig. 6

a Relative LFQ intensities for granzyme B in PBS and mefloquine-treated WT and serglycin−/− (SG−/−) mast cells. WT and SG−/− mast cells were incubated ± mefloquine (20 μM) and after 1 h cells were harvested and subjected to proteomics analysis. The data shown represent means ± SEM (n = 4). b Effect of granzyme B on mefloquine-induced ROS generation in mast cells. Mast cells were preincubated ± granzyme B inhibitor IV (GnzB inh; 10 μM) for 2 h, followed by incubation of cells ± mefloquine (20 μM). ROS levels were assessed by flow cytometry after 30 min. The data shown represent means ± SEM (n = 3)