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. 2019 May 15;9:7417. doi: 10.1038/s41598-019-43884-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AER-270 protects AQP4-sufficient but not AQP4-deficient T cells from lysis in a water swelling assay. T cells were isolated and purified from either wildtype (A) or AQP4^−/− (B) mice and cultured for 30 minutes in media only or in media containing 0.25 μM AER-270, 1.0 μM AER-270, or 0.25 mM PCMB. After pretreatment, all media was removed, and the cells were exposed to PBS or water for 5 minutes followed by the addition of 100 μl 1x PBS for controls or 2x PBS containing 10 μM calcein-AM. Fluorescence intensity was quantified and the results shown as calcein fluorescence - mean of the fluorescence of wells containing media alone (n = 5–19). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns = no significant change via a one-tailed Student t-test.