Figure 5.

Following AER-271 treatment, the changes in T cell trafficking are consistent with the decreased KLF2 expression. (A) mRNA expression of KLF2 by qRT-PCR. T cells were isolated from naive B6 mice and cultured for up to 6 hours in media only (black) or in media containing 0.25 μM AER-270 (red). Gene expression was normalized against mRNA obtained from freshly isolated, untreated spleen T cells (N = 3, repeat experiment performed 3 times with similar results observed). (B–E) Naïve B6 mice were injected with 10 mg/kg AER-271 i.p. every 6 hours for 5 days and then sacrificed on day 5. T cell numbers in the liver (B), inguinal lymph nodes (C), bone marrow (D) and the thymus (E) were determined by flow cytometry. The analysis of thymocytes included separate enumeration of double negative (DN), CD4⁺ single positive (CD4), CD8⁺ single positive (CD8) and double positive (DP) cells (E). AER-271 treated mice (red) were compared to PBS treated controls (black) (N = 4 animal/group). (*p < 0.05, **p < 0.01, ***p < 0.001, no notation indicates no significant change via a one-tailed Student t-test).