Fig. 3.

SALL3 knockdown accelerates cardiac differentiation. a Schematic diagram of culture procedures for cardiomyocyte. b qRT-PCR analysis of cardiomyocyte markers GATA4, NKX2.5, and TNNT2. Total RNA was isolated from 253G1 SALL3 shRNA cell derived and 253G1 control shRNA cell-derived cardiomyocytes (n = 3, biological replicates). ^*P < 0.01, two-sided t test. c Flow cytometry analysis of TNNT2 in 253G1 SALL3 shRNA cell-derived (right) and 253G1 control shRNA cell-derived (left) cardiomyocytes. d SALL3 mRNA levels in undifferentiated 253G1 SALL3 overexpressing cells (EF1α-SALL3). n = 3, biological replicates. ^*P < 0.01, two-sided t test. e qRT-PCR analysis of cardiomyocyte markers GATA4, NKX2.5, and TNNT2. Total RNA was isolated from 253G1 EF1α-SALL3 cell-derived and 253G1 control vector cell-derived cardiomyocytes (n = 3, biological replicates). ^*P < 0.01, two-sided t test. Error bars represent mean ± SD