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. 2019 May 15;9:7414. doi: 10.1038/s41598-019-43576-x

Figure 1.

Figure 1

UUO-induced renal interstitial fibrosis is accompanied by renal fibroblast accumulation and proliferation. (a) Representative Mallory Azan-stained kidney sections following UUO (magnification x 200). Bars, 100 μm. (b) Relative mRNA levels of Col1a1 in kidneys (n = 5 mice/group). ΔΔCT method was used to calculate relative gene expression of Col1a1 with GAPDH being the internal control. Data are expressed as mean ± SEM. (c) Accumulation of proliferating fibroblasts (GFP+PCNA+) ten days after UUO. GFP-stained renal sections were obtained from COL-GFP mice. Representative tissue sections stained with anti-GFP antibody/anti-PCNA antibody are shown. Bars, 100 μm. (d) Numbers of GFP+ cells in the kidney are expressed as the mean number ± SEM per HPF (n = 5 mice/group). (e) Numbers of renal GFP+PCNA+ cells (proliferating fibroblasts) are expressed as mean number ± SEM per HPF. (f) Percentages of renal fibroblasts that are proliferating (GFP+PCNA+ cells/total GFP+ cells).