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. 2019 May 15;9:7414. doi: 10.1038/s41598-019-43576-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ATX protein levels in the cortex of ligated kidneys increase with the progression of renal interstitial fibrosis. (a,b) Relative mRNA levels of ATX in kidneys obtained from cortex (a) and medulla (b) at day 0, 5 and 10 post-UUO (n = 5 mice/group). ΔΔCT method was used to calculate relative gene expression of ATX with GAPDH being the internal control. Data are expressed as mean ± SEM. (c,d) The expression of ATX protein in kidney lysates obtained from cortex (c) and medulla (d) at day 0, 5 and 10 post-UUO. Quantification was performed with Image J software and data are expressed as mean dots of ATX bands relative to dots of GAPDH bands ± SEM (n = 5 mice/group).