Figure 6.

ATX-LPA₁ signaling regulates renal fibroblasts migration, proliferation and myofibroblast differentiation. (a) The addition of ATX and LPC stimulated the proliferation of renal fibroblasts in an ATX dose-dependent manner. (n = 3 cell preparations/group). (b) Renal fibroblast proliferation induced by the addition of ATX with LPC was inhibited by 5 nM PAT-048 and 10 nM AM152, respectively. Data are expressed as mean ± SEM. (c,d) αSMA expression in renal fibroblasts induced by the addition of ATX with LPC was inhibited by 50 nM PAT-048 (c) and 100 nM AM152 (d) (n = 3 cell preparations/group). ΔΔCT method was used to calculate relative gene expression of αSMA with β2MG being the internal control. Data are expressed as mean ± SEM.