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. 2019 May 15;9:7430. doi: 10.1038/s41598-019-43834-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PEG2000-Ado NPs and adenosine decrease IL-1β induced NF-kB nuclear translocation in primary murine chondrocytes. Primary murine chondrocytes were cultured in the absence (Control) or presence of IL-1β and either adenosine or adenosine conjugated nanoparticles for 24 hours at 37 °C. Nuclear fractions were isolated, as described, and proteins isolated. After separation of proteins by electrophoresis through 10% polyacrylamide gels the proteins were then transferred to a nitrocellulose membrane, as described. Nuclear NFkB and P84 (a control nuclear protein) were detected following decoration with appropriate antibodies and quantitated by densitometry of bands on Western Blot. Data are expressed as % control levels. Each point represents results from an individual experiment (*p < 0.05; ***p < 0.001; n = 4–7).