Fig. 2.

sMG increases Nuclear Height and Decrease LINC elements Sun-2 and Nesprin-2G. a sMG treatment reduced apical F-actin (−37%, NS) and resulted in 76% (p < 0.01) increase in nuclear height and 52% (p < 0.05) decrease in nuclear area (n = 15/group). Apical F-actin fibers appeared disorganized in sMG groups (see Fig. S2). b Quantifying the mRNA expression of nuclear structure element LaminA/C (Lmna) and LINC elements, Sun-1, Sun-2, Nesprin-1G, and Nesprin-2G showed 31% (p < 0.05, n = 9/group) and 37% (p < 0.05, n = 9/group) decrease in Sun-2 and Nesprin-2G. Lmna and Sun-1 did not show a significant change. Nesprin-1G was not detected. c Protein levels of laminA/C and Sun-2 in SMG treated groups were lower than controls while Sun-1 showed no difference. Group comparisons were made using Mann–Whitney U-test (Fig. 2a) and One-way ANOVA followed by a Newman–Keuls post hoc test (b). *p < 0.05, **p < 0.01, ***p < 0.001, against control and each other