Fig. 3.

LIV rescue of sMG-induced proliferation loss requires LINC complex. a sMG decreased cell count by 44% after 3 days (p < 0.01, n = 8/group). LIV rescued this phenotype and increased cell count to 90% control (ns, n = 8/group). b Disabling of LINC function via siRNA against Sun-1 and Sun-2 proteins decreased the basal cell proliferation by 42% (p < 0.01, n = 8/group) addition of sMG did not decrease the cell proliferation further and LIV was unable to rescue this this decrease in both sMG and non-rotated groups. c After 3 days of sMG, YAP levels were decreased by 49% when compared to controls (p < 0.01, n = 3/group). Application of LIV increased the YAP levels by 40% when compared with sMG alone (p < 0.01, n = 3/group). Difference between Ctrl and sMG + LIV groups were not statistically significant. Group comparisons were made using One-way ANOVA followed by a Newman–Keuls post hoc test (a–c). *p < 0.05, **p < 0.01, ***p < 0.001, against control