Fig. 4.

sMG does not alter acute mechanoresponse to LIV. Following 3 days of sMG, acute mechanoresponse was assessed immediately after LIV by measuring Akt phosphorylation (Ser473) while a non-LIV control served as referent. sMG group showed a 75% increase in pAkt (p < 0.001, n = 4/group). In sMG + LIV group samples were taken off the sMG device and was subjected to daily LIV regimen (2 × 20 min, 0.7 g, 90 Hz, separated by 2 h), pAkt levels were increased by 48% when compared with non-vibrated control (p < 0.05, n = 4/group). Ground control was not subjected to sMG or LIV and showed a 93% increase in pAkt following single LIV regimen (p < 0.01, n = 4/group). No difference was found between sMG, sMG + LIV, and ground treatments. Black dashed lines correspond to non-LIV controls and their combined deviation. Group comparisons were made using One-way ANOVA followed by a Newman–Keuls post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, against control and each other