Fig. 5.

LaminA/C, Sun-2 levels, and FAK phosphorylation decreased by sMG, were recovered by LIV. We tested the effect of LIV on the structural proteins of nucleus and focal adhesion. sMG, decreased the amount of nuclear proteins LaminA/C (−67%, p < 0.01), LaminB (−41%, p < 0.01), and Sun-2 (−66%, p < 0.001) while Sun-1 showed no change. Both focal adhesion protein Vinculin and focal adhesion kinase phosphorylation at Tyr 397 residue (pFAK), indicative of integrin engagement, were decreased in response to sMG by 46 and 51% (P < 0.05). Addition of daily LIV regimen partially recovered levels of Lamin/AC (+105%, p < 0.01), Sun-2 (+36%, p < 0.01), and p-FAK (+23%, p < 0.05), but did not change LaminB, Sun-2, and Vinculin. n = 3/group for all experiments. Group comparisons were made One-way ANOVA followed by a Newman–Keuls post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, against control and each other