Skip to main content
. 2019 May;130:184–196. doi: 10.1016/j.yjmcc.2019.04.001

Fig. 7.

Fig. 7

Applications of the ratiometric SRB assay. (A) Time course of hypertrophic response to 10 μM PE applied for the final 1–24 h of incubation (in 100 μM ascorbate). Right axis: myocytes per field of view. Control had no PE added (n = 16/4). (B) Cell growth triggered by 24 h or (C) 48 h treatment with PE (n = 16/4). Best-fit to R0 + Rhyp × ([PE]n)/(EC50n + [PE]n); R0 is baseline, Rhyp is maximal increase, n is cooperativity, EC50 is the concentration at half-maximal effect. 24 h treatment: 0.266, 0.174, 0.50, 0.185 μM; 48 h treatment: 0.289, 0.223, 0.67, 0.844 μM. (D) Growth triggered by 48 h treatment with Iso; repeated in the presence of calyculin-A (5 nM) (n = 16/4). (E) Screen for anti-hypertrophic effects of drugs (inhibitors of pHi-regulating proteins) on 10 μM PE-induced cell growth (n = 25/5). DMA: 5-(N,N-dimethyl)amiloride, AR-C: AR-C155858. (F) Concentration dependence of anti-hypertrophic effect of S0859 on NRVMs stimulated for 48 h with 10 μM PE. Experiments performed on cells obtained from 5 isolations of 10–24 rat pups, with four technical repeats each. Mean ± SEM.