FIG 1.

Generation of DBPII gene fragment library. DNA gels of DBPII Sal1 digested to make gene fragments. (A) The optimal DNase I concentration was evaluated to obtain the broadest spread of DBPII fragments. Lane M contains markers. (B) DBPII Sal1 fragments obtained by digestion with 5 U/ml of DNase I and blunt ended ligated into the pHENH6 phagemid vector and transformed into E. coli TG1. (C) Sequencing of PCR products generated by screening 40 individual clones revealed that the gene fragments in the library spanned the entire coding sequence, with no bias toward any particular region. The different colors represent the lengths of the various gene fragments identified.