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. 2019 Mar 15;27(5):986–998. doi: 10.1016/j.ymthe.2019.03.007

Figure 3.

Figure 3

NGS Assessment for the Presence of Indels in Potential Off-Target Sites

PCR amplicons for 244 predicted off-target sites sequenced with at least 1,000× depth, as well as the on-target region, were analyzed (x axis, putative off-target sites arranged according to the frequency of sequence variation). The 50 highest ranking sites are represented. The first point corresponds to the on-target site (y axis, percentage of reads containing sequence variations within a 6-bp window centered on the target site for each sgRNA).