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. 2019 Feb 15;27(5):1051–1065. doi: 10.1016/j.ymthe.2019.02.009

Figure 5.

Figure 5

TGF-β1 Induced the Elevation of miR-34a and Downregulation of Klotho in HK-2 Cells

(A) qRT-PCR analysis of miR-34a expression in the HK-2 cells treated with different concentrations of TGF-β1 for 24 h. (B) qRT-PCR analysis of miR-34a expression in the HK-2 cells treated with 10 ng/mL TGF-β1 at different time. (C) qRT-PCR analysis of miR-34a expression in the HK-2 cells transfected with miR-34a inhibitor or control inhibitor. (D and E) qRT-PCR (D) and western blotting (E) analysis of Klotho in HK-2 cells after transfection with miR-34a inhibitor or control inhibitor followed by incubation with 10 ng/mL TGF-β1 for 48 h. (F) Western blotting analysis of E-cadherin, α-SMA, and fibronectin in HK-2 cells after transfection with miR-34a inhibitor or control inhibitor followed by incubation with 10 ng/mL TGF-β1 for 48 h. Data are means ± SD. ***p < 0.001, **p < 0.01, *p < 0.05.