Fig. 2.

6-MSA formation with strain CEN.PK2–1C carrying the empty vectors pJHV7 and pRS62H as control (green circles), expressing ^PpvarMSAS and native npgA from multi-copy plasmids pJHV17 and pJHV20 (red squares) and expressing ^PpvarMSAS and codon-optimized ^optnpgA from multi-copy plasmids pJHV17 and pJHV2 (blue triangles). Cultures were inoculated at low OD (0.1) and cultivated for 144 h at 30 °C in 25 mL YPD supplemented with G418 and hygromycin. 6-MSA concentrations were determined in the supernatants. Error bars represent the standard deviation of biological duplicates. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)