Fig. 5.

6-MSA uptake and conversion. A) 6-MSA consumption and B) m-cresol production of CEN.PK2–1C expressing 6-MSA decarboxylase ^optpatG from multi-copy plasmid pJHV13 or carrying empty vector pJHV7 as reference. Strains were cultivated for 72 h in YPD plus G418 with and without supplementation of 1 mM 6-MSA with an initial OD of 0.2.6-MSA and m-cresol concentrations were determined in the supernatants. Error bars represent standard deviation of biological duplicates. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)