Fig. 7.

Increase in m-cresol production by genomic integration of the pathway genes. Growth (A) and production of 6-MSA (B) and m-cresol (C) of CEN.PK2–1C expressing ^PpoptMSAS,^optnpgA and ^optpatG from multi-copy plasmid pJHV53 (blue) or from genome (strain JHY162; red). As control, the empty vector pRS42K was transformed into CEN.PK2–1C (black). High-OD fermentations (starting OD = 6) were performed in biological duplicates at 30 °C in YPD supplemented with G418 for plasmid maintenance (error bars represent standard deviations). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)